African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6860

Full Length Research Paper

Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers

Aseel K. AL-Anbari
  • Aseel K. AL-Anbari
  • Department of Biology, College of Pure Science, Diyala University, Iraq
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Nantawan Kanawapee
  • Nantawan Kanawapee
  • Applied Taxonomic Research Center, Department of Biology, Faculty of Science Khon Kaen University, Thailand.
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Talib A. AL-Kazragi
  • Talib A. AL-Kazragi
  • Department of Biology, College of Education, Tikrit University, Iraq.
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Athia Al-Mashhadani
  • Athia Al-Mashhadani
  • Department of Biology, College of Pure Science, (Ibn- al hathem), Baghdad University, Iraq.
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Sahapat Barusrux
  • Sahapat Barusrux
  • Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Thailand
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Pimwadee Pornpongrungrueng
  • Pimwadee Pornpongrungrueng
  • Applied Taxonomic Research Center, Department of Biology, Faculty of Science Khon Kaen University, Thailand.
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PiyadaTheerakulpisut
  • PiyadaTheerakulpisut
  • Applied Taxonomic Research Center, Department of Biology, Faculty of Science Khon Kaen University, Thailand.
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  •  Accepted: 20 February 2014
  •  Published: 14 March 2014

Abstract

Citrus is an economically important fruit crop with a long history of cultivation worldwide. A great number of varieties of citrus are extensively grown in the Middle East including Iraq for domestic consumption and exports. However, the genetic diversity of this genus in Iraq has not been reported. Therefore, the objective of this study was to evaluate genetic relationships of Iragi citrus genotypes to provide useful information for germplasm conservation and planning of breeding strategies. Twenty decamer primers were used to generate RAPD markers to evaluate genetic relationship among 16 genotypes (14 species and hybrids) of cultivated Citrus in Iraq. Based on RAPD polymorphisms, the citrus genotypes were classified into two main groups; the first consisted of citron (Citrus medica) and its hybrids (lime and lemon). The second group contained the remaining genotypes including three sub-groups; the first being formed of sour orange (Citrus aurantium), sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi), the second the mandarins (Citrus reticulata) and the third the pummelo (Citrus grandis). The RAPD-based classification was consistent with previous studies based on other types of molecular markers.

 

Key words: Citrus, genetic diversity, Iraq, oranges, random amplified polymorphic DNA (RAPD) markers. 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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