African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Simultaneous detection of three transgenic events in crops using multiplex polymerase chain reaction (PCR)

Mariana Cantú-Iris1, Claudia Macías-de-la-Cerda1, Jesús Morlett Chávez1,2, Raúl Rodríguez- Herrera1,4*, Cristóbal Noé Aguilar1 and Humberto Reyes-Valdés3
1Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, MX-25280 Coahuila, México. 2Molecular Diagnosis and Clinical Analyses Laboratory, Universidad Autónoma de Coahuila, Saltillo, MX-25280 Coahuila, México. 3Plant Breeding Department, Universidad Autónoma Agraria Antonio Narro, Buenavista, Saltillo, MX-25000 Coahuila, México. 4Blvd. V. Carranza e Ing. José Cárdenas V. s/n. Col. República Ote. C.P. 25280. Saltillo, Coahuila. México.
Email: [email protected]

  •  Accepted: 30 January 2012
  •  Published: 26 February 2012

Abstract

In spite of all advantages for using end-point polymerase chain reaction (PCR) this technique can onlyshows a limiting: only is possible to detect one gene DNA sequence by reaction. However multiplex PCR has been proposed for employing in the detection of two or more DNA sequences or genes. This implies sometimes savinge time and money. The objective of this study was to adapt the multiplex PCR technique for detection off the either two or three of the most common transgenic genes present in different plants. The DNA was isolated from maize, soybean and cotton plants. The optimization of the multiplex PCR was performed changing the concentration of MgCl2, primer annealing temperature and primer design. With this optimization it was possible to amplify three genes (cry 1Ab, epsps and als) in same reaction of PCR. These results may lead to icreasedincreased efficienceefficiency in have an impact more efficient on detection of genetically modified organisms.

 

Key words: Multiplex polymerase chain reaction (PCR), cry 1Ab, epsps, als.