Full Length Research Paper
In spite of all advantages for using end-point polymerase chain reaction (PCR) this technique
shows a limiting: only is possible to detect one gene DNA sequence by reaction. However multiplex PCR has been proposed for employing in the detection of two or more DNA sequences or genes. This implies sometimes sav e time and money. The objective of this study was to adapt the multiplex PCR technique for detection o f the either two or three of the most common transgenic genes present in different plants. The DNA was isolated from maize, soybean and cotton plants. The optimization of the multiplex PCR was performed changing the concentration of MgCl2, primer annealing temperature and primer design. With this optimization it was possible to amplify three genes (cry 1Ab, epsps and als) in same reaction of PCR. These results may have an impact more efficient on detection of genetically modified organisms.
Key words: Multiplex polymerase chain reaction (PCR), cry 1Ab, epsps, als.
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