African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

The biochemical constituents and pectinase activities associated with the virulence of Rhizoctonia solani isolates in rice in West Bengal, India

A. Mondal1*, S. Dutta2, S. P. Kuiry2, D. Chakraborty3, S. Nandi2, S. Das2, S. K. Ray2 and S. Chaudhuri1
1Department of Botany, University of Kalyani, Kalyani, Nadia, West Bengal - 741235, India. 2Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal-742152, India. 3Department of Plant Physiology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal-742152, India.
Email: [email protected], [email protected]

  •  Accepted: 11 June 2013
  •  Published: 20 June 2013

Abstract

Twenty five isolates of Rhizoctonia solani were isolated from infected rice fields from different agro-ecological regions of West Bengal. Cellulase and pectinase activities of the isolates were detected using carboxymethylcellulose (CMC) and pectin agar media, respectively. Diameter of the clearing zones on the respective media represented the level of enzyme activity of each isolate. Mycelial protein, carbohydrate and phenol contents were also recorded. The virulence of the isolates was studied on the rice cultivar (MTU 7029) under greenhouse conditions. Correlation of the biochemical constituents and enzyme activity with virulence of the R. solani isolates was performed. Pectinase activity found to be the important factor could predict the variation in the virulence of R. solani strains up to seventy six percent.

 

Key words: Pectinase, mycelial protein, carbohydrate, virulence, Rhizoctonia solani.