African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6842

Full Length Research Paper

Biochar as an alternative to improve the in vitro environment for Pitaya (Hylocereus undatus Haw) and strawberry (Fragaria x ananassa Duch) growing

Laura Souza Santos
  • Laura Souza Santos
  • Centro de Ciências Agrárias, Universidade Estadual de Londrina, 86055-900, Londrina, PR, Brazil.
  • Google Scholar
Érika Manuela Gonçalves Lopes
  • Érika Manuela Gonçalves Lopes
  • Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, 39404-547, Montes Claros, MG, Brazil.
  • Google Scholar
Claudinéia Ferreira Nunes
  • Claudinéia Ferreira Nunes
  • Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, 39404-547, Montes Claros, MG, Brazil.
  • Google Scholar
Demerson Arruda Sanglard
  • Demerson Arruda Sanglard
  • Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, 39404-547, Montes Claros, MG, Brazil.
  • Google Scholar
Eulina Fernandes Damião
  • Eulina Fernandes Damião
  • Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, 39404-547, Montes Claros, MG, Brazil.
  • Google Scholar
Matheus Mendes Reis
  • Matheus Mendes Reis
  • Instituto Federal do Norte de Minas Gerais, 39480-000, Januária, MG, Brazil.
  • Google Scholar
Leidivan Almeida Frazão
  • Leidivan Almeida Frazão
  • Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, 39404-547, Montes Claros, MG, Brazil.
  • Google Scholar
Luiz Arnaldo Fernandes
  • Luiz Arnaldo Fernandes
  • Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, 39404-547, Montes Claros, MG, Brazil.
  • Google Scholar


  •  Received: 19 October 2022
  •  Accepted: 18 January 2023
  •  Published: 31 March 2023

Abstract

In vitro cultivation is widely used for micropropagation of several plant species; however, the increase in the gas concentration, such as ethylene gas, inside the growth vials can cause physiological changes. Thus, 4 experiments were carried out at the Federal University of Minas Gerais to evaluate the biochar use from sugarcane bagasse in vials with or without caps with porous membrane in the in vitro growing of pitaya (Hylocereus undatus Haw) (experiment 1) and strawberry (Fragaria x ananassa Duch) (experiment 2) and evaluating in combination of the use of biochar from sugarcane bagasse and AgNO3 in the in vitro growing of pitaya (experiment 3) and strawberry (experiment 4). The types of caps did not affect the growth and number of pitaya and strawberry; however, using caps with porous membrane obtained more magnificent roots dry matter yield. The addition of 4 g L-1 of biochar, regardless of cap type, favored the pitaya and strawberry roots' growth and development. AgNO3 alone contributed to the higher length and dry mass of pitaya roots and greater dry mass of strawberry roots. Adding biochar decreases the AgNO3 effects in vitro culture. Biochar is an alternative to improve the in vitro environment of plant species.

Key words: Fragaria x ananassa Duch, Hylocereus undatus Haw, Tissue culture, AgNO3, gas exchange.