African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Genetic relationships among Jatropha curcas L. clones from Panzhihua, China as revealed by RAPD and ISSR

Ke Chen1,2, Peng Ren2, Chunyin Ying2*, Qi Jiang3 and Xiuqing Jia1
1School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Peoples Republic of China. 2Chengdu Institute of Biology, Chinese Academy of Sciences, P. O. Box 416, Chengdu 610041, Peoples Republic of China. 3Panzhihua of Sichuan Provincial Academy of Agroforestry Sciences, Panzhihua 617061, Peoples Republic of China.
Email: [email protected]

  •  Accepted: 11 May 2011
  •  Published: 04 June 2011

Abstract

Although Jatropha curcas L. is known as a source of oil-rich seeds as economically viable substitute of diesel, little is currently known about the genetic resource of J. curcas in China. In this study, RAPD and ISSR data were utilized to determine the genetic relationships among J. curcas clones from Panzhihua, China. Five RAPD and twelve ISSR primers generated reproducible amplification banding pattern of 43 polymorphic bands out of 126 scored accounting, for 34% polymorphism across the clones. The UPGMA cluster analysis indicated two broad groups, one comprising all the clones except the clones from YuanMou, which formed the smaller second group. Congruously, the samples belonging to the same origin grouped into their distinct origin lineages. The overall grouping pattern of clustering was irrelevant to the seeds oil content of these clones. This study provides valid guidelines for collection, conservation and characterization of J. curcas genetic resources from Panzhihua and also for further breeding program towards biodiesel production.

 

Key words: Jatropha curcas L., genetic relationship, molecular marker.