African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6860

Full Length Research Paper

Molecular analysis of genetic diversity in olive cultivars

  Azmat Ali Awan1*, Muhammad Zubair1, Aqib Iqbal2, Sayed Jaffar Abbas3 and Naushad Ali4  
  1Horticulture Department, Khyber Pakhtunkhwa, Agriculture University, Peshawar, Pakistan. 2Institute of Biotechnology and Genetic Engineering, Khyber Pakhtunkhwa, Agriculture University, Peshawar, Pakistan. 3Biotechnology Center, Agriculture Research Institute, Tarnab, Peshawar, Pakistan. 4Plant Breeding Department, Khyber Pakhtunkhwa, Agriculture University, Peshawar, Pakistan.  
Email: [email protected]

  •  Accepted: 09 August 2011
  •  Published: 05 October 2011

Abstract

 

The present study was conducted to study the genetic diversity in the available cultivars of olive flora in Pakistan. On an average, 15.5 alleles were amplified using Randomly Amplified Polymorphic DNA (RAPD) molecular primers. Mean genetic distance estimates ranged from 0.158 to 0.512 (G.D = 15.8 to 51.2%). Size of scorable fragments ranged from approximately 250 to >2000 bp. A high level of genetic dissimilarity (GD= up to 51%) was recorded among Ferugenia genotype only. Entries were grouped in clusters using cluster analysis. On the basis of dendrogram, most diverse genotypes Ferugenia and Coratina were identified, that can be used in future olive propagation program.

 

Key words: Olive, randomly amplified polymorphic DNA (RAPD) molecular primers, genetic diversity, Pakistan.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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