African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6863

Full Length Research Paper

In vitro inhibitory effect of selected fungicides on mycelial growth of ambrosia fungus associated with the black coffee twig borer, Xylosandrus compactus Eichhoff (Coleoptera: Curculionidae) in Uganda

Godfrey Kagezi
  • Godfrey Kagezi
  • Coffee Research Center (COREC)/National Crops Resource Research Institute (NaCRRI)/National Agricultural Research Organization (NARO). P. O. Box 185 Mukono, Uganda
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Patrick Kucel
  • Patrick Kucel
  • Coffee Research Center (COREC)/National Crops Resource Research Institute (NaCRRI)/National Agricultural Research Organization (NARO). P. O. Box 185 Mukono, Uganda
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Sammy Olal
  • Sammy Olal
  • Coffee Research Center (COREC)/National Crops Resource Research Institute (NaCRRI)/National Agricultural Research Organization (NARO). P. O. Box 185 Mukono, Uganda
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Fabrice Pinard
  • Fabrice Pinard
  • International Centre of Insect Physiology and Ecology (ICIPE), CIRAD, Plant Health Department P. O. Box 30772 - 00100, Nairobi, Kenya.
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Joseph Seruyange
  • Joseph Seruyange
  • Faculty of Biological Science, Kyambogo University. P. O. Box 1 Kyambogo, Uganda.
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Pascal Musoli
  • Pascal Musoli
  • Coffee Research Center (COREC)/National Crops Resource Research Institute (NaCRRI)/National Agricultural Research Organization (NARO). P. O. Box 185 Mukono, Uganda
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Africano Kangire
  • Africano Kangire
  • Coffee Research Center (COREC)/National Crops Resource Research Institute (NaCRRI)/National Agricultural Research Organization (NARO). P. O. Box 185 Mukono, Uganda
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  •  Received: 15 August 2012
  •  Accepted: 18 December 2013
  •  Published: 04 June 2015

Abstract

Black coffee twig borer is a new but rapidly spreading insect pest of coffee in Uganda. Female beetles bore into primary branches/twigs and cultivate an ambrosia fungus for feeding their larvae. Thus, controlling the fungus means depriving the brood a source of food. Three fungicides, chlorothalonil (Glider), tebuconazole (Orius 25EW) and dimethomorph + mancozeb (Volar) were evaluated in vitro for their effectiveness in inhibiting mycelial growth of ambrosia fungus associated with the beetle. The pathogen was exposed to four concentrations (1.5x, 1.25x, 1.0x and 0.5x times the manufacturer recommended rate) incorporated into potato dextrose agar using inhibition and food poisoning techniques. The three fungicides inhibited fungal growth to some extent, even at the lowest concentration (0.5x) and percentage inhibition was significantly different (P≤0.05) from each other. Tebucozanole caused 100% growth inhibition irrespective of concentration and technique used while chlorothalonil and dimethomorph + mancozeb caused less than 40% inhibition for both techniques. Therefore, research should determine effectiveness of tebucozanole for suppressing fungal growth under field conditions for diminishing beetle incidence and fungal pathogenic effects in infested branches. This will pave way for integration of use of tebucozanole into overall Integrated Pest Management package (IPM) for the beetle in Uganda. 

 

Key words:       Ambrosia-fungus, black-coffee-twig-borer, chlorothalonil, dimethomorph + mancozeb, fungicides, tebuconazole, Xylosandrus-compactus.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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