Pollen tubes transport nuclei to the ovules for fertilization. The distribution of microfilaments and the nuclei were investigated by fluorescent phalloidin labeling and DAPI (4', 6-diamidino-2- phenylindole) during pollen germination and pollen tube growth of Populus tomentosa Carr., a Chinese native tree species. The coexistence of trinucleate and binucleate pollen was confirmed, which had been previously considered binucleate. Three distinct typical microfilament structures were found, and F-actin was present at the periphery of both the vegetative and the sperm nuclei. The investigation indicated that movement of sperm nuclei and vegetative nucleus is related to the microfilament system.
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