African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6730

Full Length Research Paper

Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia

Tatjana Popović1, Jelica Balaž2, Zorica Nikolić3, Mira Starović1, Veljko Gavrilović1, Goran Aleksić1, Mirjana Vasić3 and Svetlana Živković1  
1]Institute for Plant Protection and Environment, Belgrade, Serbia. 2Faculty of Agriculture, Novi Sad, Serbia. 3Institute for Vegetable and Field Crops, Novi Sad, Serbia
Email: [email protected]

  •  Accepted: 23 September 2010
  •  Published: 04 October 2010


Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.


Key words: Common bacterial blight, detection, identification, seed-borne.