African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area

Sara Jelodarian
  • Sara Jelodarian
  • Islamic Azad University, Science and Research Branch, Faculty of Agriculture and Natural Sources, Tehran, Islamic Republic of Iran.
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Abdolrasoul Ebrahimabadi
  • Abdolrasoul Ebrahimabadi
  • Essential Oils Research Institute, University of Kashan, Post Code 87317-51167 Kashan, Islamic Republic of Iran.
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Ahmad Khalighi
  • Ahmad Khalighi
  • Islamic Azad University, Science and Research Branch, Faculty of Agriculture and Natural Sources, Tehran, Islamic Republic of Iran.
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Hossain Batooli
  • Hossain Batooli
  • Isfahan Research Center of Natural Sources and Agriculture, Kashan Station, Kashan, Islamic Republic of Iran.
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  •  Received: 18 January 2012
  •  Accepted: 26 April 2015
  •  Published: 14 May 2015

Abstract

Antioxidants are considered as the main factors in the inhibition of unwanted oxidation reactions. In this research the antioxidant potential of the fresh fruits of 4 cultivars of Malus domestica cultivated in the Kashan, Qamsar area was evaluated. The antioxidant activity of the samples were evaluated using two complementary antioxidant assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid tests and the results were compared with the synthetic standard antioxidant butylated hydroxytoluene (BHT). Total phenolic contents of the samples are also estimated by Folin-Ciocalteu's phenol test. In both DPPH β-carotene/linoleic acid tests in the concentration of 2 mg/ml, only samples from Hossain cultivar showed moderate antioxidant activity with 63.92 ± 0.42 and 6.02 ± 0.03 inhibition percentages, respectively and other samples were only weekly active. The Folin-Ciocalteu's phenol test was also showed very little phenolic compounds for the fruits. In conclusion, week antioxidant activity was estimated for the studied apple cultivars.

 

Key words: Apple, Malus domestica, extract, antioxidant activity, total phenolic content.