African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Short Communication

Comparative analysis of volatile constituents in Bacillus thuringiensis (Bt) cotton (RCH2 Bt) and non-Bt cotton by gas chromatography-mass spectrometry

Palanisamy Parimala, Subbiah Ramalakshmi and Krishnaswamy Muthuchelian*
Department of Bioenergy, School of Energy, Environment and Natural resources, Madurai Kamaraj University, Madurai, India.
Email: [email protected]

  •  Accepted: 15 October 2013
  •  Published: 24 October 2013

Abstract

The differences in composition and concentrations of volatile allelochemicals between transgenic Bacillus thuringiensis (Bt) cotton and non Bt cotton leaves were compared and analyzed by using gas chromatography-mass spectrometry (GC-MS). The dominant compounds present in both non Bt and Bt cotton leaves were α-caryophyllene (4.62, 1.42), β-myrcene (3.67, 3.68), β-carene (0.31, 0.80), benzene acetaldehyde (0.88, 0.48), heptanoic acid (2.0, 0.67), 1-methoxy, 2-methyl benzene (0.67, 0.30) and acetic acid, phenyl methyl ester (4.32, 2.48). The differences in main components and ratios of volatile allelochemics between Bt cotton and non Bt cotton suggested that, the expression of Bt toxin protein in cotton was found to have an impact on their volatile profiles.

 

Key words: Bt cotton (RCH2 Bt), non-Bt cotton, gas chromatography-mass spectrometry (GC-MS).