African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6849

Full Length Research Paper

Diversity and distribution of arbuscular mycorrhizal fungi in maize (Zea mays) cropping fields in South Kivu, Democratic Republic of Congo

Réussite Bugale Malembaka
  • Réussite Bugale Malembaka
  • Department of Land Resource Management and Agricultural Technology, Faculty of Agriculture, University of Nairobi, Kenya.
  • Google Scholar
Richard Onwonga
  • Richard Onwonga
  • Department of Land Resource Management and Agricultural Technology, Faculty of Agriculture, University of Nairobi, Kenya.
  • Google Scholar
Joyce Jefwa
  • Joyce Jefwa
  • Mycology Laboratory, Botany Department, East Africa Herbarium, Kenya
  • Google Scholar
Fredrick Ayuke
  • Fredrick Ayuke
  • Department of Land Resource Management and Agricultural Technology, Faculty of Agriculture, University of Nairobi, Kenya.
  • Google Scholar
Leon Nabahungu
  • Leon Nabahungu
  • International Institute of Tropical Agriculture, Olusegun Obasanjo Station, DR Congo.
  • Google Scholar

  •  Received: 09 December 2020
  •  Accepted: 09 February 2021
  •  Published: 30 April 2021


Alexandra E (2017). Effect of Arbuscular Mycorrhiza Fungi on the crop production in Katanga Democratic Republic of the Congo. Dissertation. Gent University. Belgium.
Augé RM (2001). Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11(1):3-42.
Badibanga T (2013). Agricultural Development in the Democratic Republic of the Congo: Constraints and Opportunities. Revue d'intelligence stratégique et des relations internationales 25 p.
Bagula ME, Pypers P, Mushagalusa NG, Muhigwa JB (2014). Assessment of Fertilizer Use Efficiency of Maize in the Weathered Soils of Walungu District, DR Congo. In Vanlauwe B Van Asten P, Blomme G. Eds. Challenges and Opportunities for Agricultural Intensification of the Humid Highland Systems of Sub-Saharan Africa. Springer pp. 187-199.
Bashagaluke JB (2014). Characterization of Soils Using Infrared Scanning in South Kivu Province, Democratic Republic of Congo. Dissertation, Kenyatta University. Nairobi.
Belay Z, Vestberg M, Assefa F (2013). Diversity and abundance of arbuscular mycorrhizal fungi associated with acacia trees from different land use systems in Ethiopia. African Journal of Microbiology Research 7(48):5503-5515.
Bona E, Scarafoni A, Marsano F, Boatti L, Copett, A, Massa N. Amalero E, D'Agostino G, Cesaro P, Cavaletto M, Berta G (2016). Arbuscular mycorrhizal symbiosis affects the grain proteome of Zea mays: a field study. Scientific Reports 6:26439.
Borriello R, Lumini E, Girlanda M, Bonfante P, Bianciotto V (2012). Effects of different management practices on arbuscular mycorrhizal fungal diversity in maize fields by a molecular approach. Biology and Fertility of Soils 48(8):911-922.
Brundrett M, Bougher N, Dell B, Grove T, Malajczuk N (1996). Working with mycorrhizas in forestry and agriculture. Canberra: Australian Centre for International Agricultural Research 34:35.
Cozzolino V, Di Meo V, Piccolo A (2013). Impact of arbuscular mycorrhizal fungi applications on maize production and soil phosphorus availability. Journal of Geochemical Exploration 129:40-44.
Crawford TW, Singh U, Breman H (2008). Résoudre les Problèmes Agricoles Relatifs à l'Acidité du Sol dans la Région des Grands Lacs de l'Afrique Centrale. International Fertilizer Development Center CATALIST, Kigali 137 p.
Crespo R (2015). Impact of arbuscular mycorrhizal fungi on the physiology of maize genotypes under variable nitrogen and phosphorus levels. Dissertations. University of Nebraska - Lincoln. USA.
Crossay T, Cilia A, Cavaloc Y, Amir H, Redecker D (2018). Four new species of arbuscular mycorrhizal fungi (Glomeromycota) associated with endemic plants from ultramafic soils of New Caledonia. Mycological Progress 17(6):729-744.
Dalpé Y, Hamel C. (2008). Arbuscular Mycorrhizae. In Gregorich, E.G. and Carter, M.R. Eds. Soil sampling and methods of analysis. CRC Press pp. 355-378.
de Oliveira Júnior JQ, da Conceição JE, Lisboa FJ, Berbara RLL, de Faria SM (2017). Nitrogen-fixing bacteria and arbuscular mycorrhizal fungi in Piptadenia gonoacantha. Brazilian Journal of Microbiology 48(1):95-100.
Eulenstein F, Tauschke M, Behrendt A, Monk J, Schindler U, Lana MA, Monk S (2016). The Application of Mycorrhizal Fungi and Organic Fertilisers in Horticultural Potting Soils to Improve Water Use Efficiency of Crops. Horticulturae 3(1):8.
Gomes EA, Oliveira CA, Lana UG, Noda RW, Marriel IE, De Souza FA (2015). Arbuscular mycorrhizal fungal communities in the roots of maize lines contrasting for Al tolerance grown in limed and non-limed Brazilian Oxisoil. Journal of Microbiolog and Biotechnology 25(7):978-987.
Habiyaremye J, Muthuri C, Matiru V, Nyaga J, Mukuralinda A, Ruganzu V, Ngabonziza JD, Sinclair F (2018). Indigenous arbuscular mycorrhizal fungi associated with tree species of the agroforestry systems of Rwanda and their potential to colonize maize roots. African Journal of Microbiology Research 12(36):879-888.
Hazelton P, Murphy B (2016). Interpreting soil test results: What do all the numbers mean? Commonwealth Scientific and Industrial Research Organisation Publishing 185 p.
Heri-kazi BA (2011). Evaluation de la diversité des sols et leur prise en compte dans la terminologie vernaculaire et les modes de gestion de l'espace agricole au Sud Kivu, cas de territoires de Walungu et Kabare. MSc dissertation. Université de Liège, Belgium.
Ingleby K (2007). Assessment of mycorrhizal diversity in soils and roots, and nursery inoculation to improve the survival and growth of seedlings. Mycorrhizal training manual. Centre for Ecology and Hydrology. PENICUIK 87 p.
Ingleby, K., Diagne, O., Deans, J. D., Lindley, D. K., Neyra, M. and Ducousso, M., (1997). Distribution of roots, arbuscular mycorrhizal colonisation and spores around fast-growing tree species in Senegal. Forest Ecology and Management 90(1):19-27.
International Culture Collection of Vesicular Arbuscular Mycorrhizal Fungi (INVAM) (2019).
IPAPEL (2011). Inspection Provinciale de l'Agriculture, Pêche et Elevage, Rapport annuel de l'année 2011. 41 p.
Jansa J, Erb A, Oberholzer HR, Šmilauer P, Egli S (2014). Soil and geography are more important determinants of indigenous arbuscular mycorrhizal communities than management practices in Swiss agricultural soils. Molecular Ecology 23(8):2118-2135.
Jefwa J, Sinclair R, Maghembe JA (2006). Diversity of glomale mycorrhizal fungi in maize/sesbania intercrops and maize monocrop systems in southern Malawi. Agroforestry Systems 67(2):107-114.
Jones Jr JB (2001). Laboratory guide for conducting soil tests and plant analysis. CRC press, USA 382 p.
Kottek M, Grieser J, Beck C, Rudolf B, Rubel F (2006). World map of the Köppen-Geiger climate classification updated. Meteorologische Zeitschrift 15(3):259-263.
Krüger M, Walker C, Schüßler A (2011). Acaulospora brasiliensis comb. nov. and Acaulospora alpina (Glomeromycota) from upland Scotland: morphology, molecular phylogeny and DNA-based detection in roots. Mycorrhiza 21(6):577-587.
Kulimushi PZ, Basime GC, Nachigera GM, Thonart P, Ongena M (2018). Efficacy of Bacillus amyloliquefaciens as biocontrol agent to fight fungal diseases of maize under tropical climates: from lab to field assays in South Kivu. Environmental Science and Pollution Research 25(30):29808-29821.
Lambert DH, Baker DE, Cole HJr (1979). The role of mycorrhizae in the interactions of phosphorus with zinc, copper, and other elements. Soil Soil Science Society of America Journal 43:976-980.
Liu B, Li H, Zhu B, Koide RT, Eissenstat DM, Guo D (2015). Complementarity in nutrient foraging strategies of absorptive fine roots and arbuscular mycorrhizal fungi across 14 coexisting subtropical tree species. New Phytologist 208(1):125-136.
MinAgri (2018). Sécurité alimentaire, niveau de production agricole et Animale, Évaluation de la Campagne Agricole 2017-2018 et Bilan Alimentaire du Pays. Rapport du Ministère de l'Agriculture 75 p.
Muhindo DI, Mwanjalolo JGM, Katushabe AA, Masamba JW, Nkuba B (2017). Projected impact of climate change on rice yield in two agro-ecological zones in South-Kivu, Democratic Republic of Congo. African Journal of Rural Development 1(3):293-304.
Nandjui J, Voko R, Kouadio ANM, Fosto B, Tano Y, Zeze A (2013). Assessment of the occurrence and abundance of mycorrhizal fungal communities in soils from yam (Dioscorea spp.) cropping fields in Dabakala, North Cote d'Ivoire. African Journal of Agricultural Research 8(44):5572-5584.
Ndonda A (2018). Evaluations agronomiques des champignons mycorhiziens locaux sur la productivité du Manioc (Manihot esculenta Crantz) en sols dégradés des jachères herbeuses à Kisangani/ R.D Congo. Dissertation. Université de Kisangani. D R Congo.
Nelson DW, Sommers LE (1975). Determination of Total Nitrogen in Natural Waters. Journal of Environmental Quality 4(4):465-468.
Ngongo ML, Van Ranst E, Baert G, Kasongo EL, Verdoodt A, Mujinya BB, Mukalay JM (2009). Guide des Sols en République Démocratique du Congo, Tome I: Etude et Gestion. Don Bosco, Lubumbashi, DR Congo 260 p.
Nwaga D, Ambassa-Kiki R, Ngonkeu Mangaptché EL, Tchiegang-Megueni C (2003). Selection of arbuscular mycorrhizal fungi for inoculating maize and sorghum grown in Oxisol/Ultisol and Vertisol in Cameroon. In Bationo, A (Ed). Managing Nutrient Cycles to Sustain Soil Fertility in Sub-Saharan Africa. Academy Science Publishers pp. 467-486.
Nziguheba G, Zingore S, Kihara J, Merckx R, Njoroge S, Otinga A, Vandamme E, Vanlauwe B (2016). Phosphorus in smallholder farming systems of sub-Saharan Africa: implications for agricultural intensification. Nutrient Cycling In Agro Ecosystems 104(3):321-340.
Oehl F, Sieverding E, Ineichen K, Mäder P, Boller T, Wiemken A (2003). Impact of land use intensity on the species diversity of arbuscular mycorrhizal fungi in agroecosystems of Central Europe. Applied and Environmental Microbiology 69(5):2816-2824.
Oehl F, Sieverding E, Palenzuela J, Ineichen K, Da Silva GA (2011). Advances in Glomeromycota taxonomy and classification. IMA Fungus 2(2):191-199.
Oehl F, Sykorova Z, Redecker D, Wiemken A, Sieverding E (2006). Acaulospora alpina, a new arbuscular mycorrhizal fungal species characteristic for high mountainous and alpine regions of the Swiss Alps. Mycologia 98(2):286-294.
Oehl F, Tchabi A, Silva GA, Sanchez-Castro I, Palenzuela J, do Monte Junior IP, Lawouin LE, Coyne D, Hountondji FC (2014). Acaulospora spinosissima, a new arbuscular mycorrhizal fungus from the Southern Guinea Savanna in Benin. Sydowia 66(1):29-42.
Okalebo JR. Gathua KW, Woomer PL (2002). Laboratory Methods of Soil and Plant Analysis: A Working Manual. 2nd Edition. Soil Science Society of East Africa. Technical Publication 1:131.
Pereira CM, Goto BT, Silva DKAD, Ferreira ACAD, Souza FAD, Silva GAD, Maia LC, Oehl F (2016). Acaulospora reducta sp. nov. and A. excavate - two glomeromycotan fungi with pitted spores from Brazil. Mycotaxon 130(4):983-995.
Quoreshi AM (2008). The use of mycorrhizal biotechnology in restoration of disturbed ecosystem. In Siddiqui Z A, Akhtar SM, Futai K. Mycorrhizae: Sustainable Agriculture and Forestry. Springer. New Dehli pp 303-320.
Redecker D, Schüßler A, Stockinger H, Stürmer SL, Morton JB, Walker C (2013). An evidence-based consensus for the classification of arbuscular mycorrhizal fungi; Glomeromycota. Mycorrhiza 23(7):515-531.
Rosendahl S (2008). Communities, populations and individuals of arbuscular mycorrhizal fungi. New Phytologist 178(2):253-266.
Santpoort R (2020). The Drivers of maize area expansion in Sub shaharan Africa. How policies to boost maize production overlook the interests of smallholder farmers. Land 9:68.
Sasvári Z, Hornok L, Posta K (2011). The community structure of arbuscular mycorrhizal fungi in roots of maize grown in a 50-year monoculture. Biology and Fertility of Soils 47:167-176.
Sasvári Z, Magurno F, Galanics D, Hang TT, Ha TT, Luyen ND, Posta K (2012). Isolation and identification of arbuscular mycorrhizal fungi from agricultural fields of Vietnam.
Sendek A, Karakoç C, Wagg C, Domínguez-Begines J, do Couto GM, van der Heijden MG, Naz A A, Lochner A, Chatzinotas A, Klotz S, Gómez-Aparicio L (2019). Drought modulates interactions between arbuscular mycorrhizal fungal diversity and barley genotype diversity. Nature Scientific Reports 9(1):1-15.
Séry D, Kouadjo ZG, Voko BR, Zézé A (2016). Selecting native arbuscular mycorrhizal fungi to promote cassava growth and increase yield under field conditions. Frontiers in Microbiology 7:2063.
Siqueira JO, Hubbell DH, Mahmud AW (1984). Effect of liming on spore germination, germ tube growth and root colonization by vesicular-arbuscular mycorrhizal fungi. Plant Soil 76(1):115-124.
Smith SE, Smith FA (2011). Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales. Annual review of plant biology 62:227-250.
Solaiman ZM, Abbott LK, Varma A (2014). Mycorrhizal fungi: use in sustainable agriculture and land restoration. Springer. Berlin 41:404.
Stürmer SL, Siqueira, J. O., 2011. Species richness and spore abundance of arbuscular mycorrhizal fungi across distinct land uses in Western Brazilian Amazon. Mycorrhiza 21(4):255-267.
Tchabi A, Coyne D, Hountondji F, Lawouin L, Wiemken A, Oehl F (2010). Efficacy of indigenous arbuscular mycorrhizal fungi for promoting white yam (Dioscorea rotundata) growth in West Africa. Applied Soil Ecology 45(2):92-100.
Teixeira AFDS, Kemmelmeier K, Marascalchi MN, Stürmer SL, Carneiro MAC, Moreira FMDS (2017). Arbuscular mycorrhizal fungal communities in an iron mining area and its surroundings: Inoculum potential, density, and diversity of spores related to soil
properties. Ciência e Agrotecnologia 41(5):511-525.
Vieira CK, Marascalchi MN, Rodrigues AV, de Armas RD, Stürmer SL (2018). Morphological and molecular diversity of arbuscular mycorrhizal fungi in revegetated iron-mining site has the same magnitude of adjacent pristine ecosystems. Journal of Environmental Sciences 67:330-343.
Walker C, Harper CJ, Brundrett MC, Krings M (2018). Looking for arbuscular mycorrhizal fungi in the fossil record?: an illustrated guide. In. Krings M, Harper CJ, Cuneo RC, Rothwell GW: Eds, Transformative Paleobotany. Academic Press, New York. pp 481-517.