African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12236

Full Length Research Paper

In vitro production of thiophenes using hairy root cultures of Tagetes erecta (L.)

Vijayta Gupta
  • Vijayta Gupta
  • Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Kukrail Picnic Spot Road, P. O. CIMAP, Lucknow 226015, India.
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Karuna Shanker
  • Karuna Shanker
  • Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Kukrail Picnic Spot Road, P. O. CIMAP, Lucknow 226015, India.
  • Google Scholar
Laiq ur Rahman
  • Laiq ur Rahman
  • Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Kukrail Picnic Spot Road, P. O. CIMAP, Lucknow 226015, India.
  • Google Scholar


  •  Received: 07 February 2015
  •  Accepted: 23 September 2015
  •  Published: 27 April 2016

Abstract

Marigold (Tagetes spp.) is a source of thiophenes, which are a group of heterocyclic sulfurous compounds possessing strong biocidal activity, thus making Tagetes plants very useful as natural source of agents for controlling pathogens such as nematodes, insects, fungi and bacteria. Hairy root cultures of Tagetes erecta L. were developed using Agrobacterium rhizogenes mediated transformation. The hairy root clones showed great variations in growth pattern and total thiophene content (0.31 to 0.96 mg/gfw). Four types of thiophenes that accumulated in root cultures of Tagetes were butenenylbithiophene (BBT), hydroxybutenenylbithiophene (BBTOH), acetoxybutenenylbithiophene (BBTOAc) and α-terthienyl (α-T). Total thiophene contents in these isolated rhizoclones were found to be four fold higher than that of wild type hairy root cultures. The developed method of producing hairy cultures of T. erecta can be used for producing thiophenes at large scale.

Key words: Agrobacterium rhizogenes, marigold, rhizoclones, biocidal.

Abbreviation

BBT, Butenenylbithiophene; BBTOH, hydroxybutenenylbithiophene; BBTOAc, acetoxybutenenylbithiophene; α-T, α-terthienyl; EPA, environmental protection agency; MeBr, methyl bromide.