African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular cloning and characterization of a new peroxidase gene (OvRCI) from Orychophragmus violaceus

Dai Qilin1,2, Wang Jin2,3, Feng Bin3, Liu Tingting3, Chen Chen4, Lin Honghui1and Du Shizhang2*
  1Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610064, China. 2Plant Genomics Research center, Mianyang Normal College, Mianyang 621000, China. 3Plant Biotechnology Research Centre, Southwest University of Science and Technology, Mianyang 621000, China. 4School of Pharmacy, Fudan University, Shanghai 200030, China.
Email: [email protected], [email protected]

  •  Accepted: 05 November 2009
  •  Published: 01 December 2009

Abstract

 

A new peroxidase gene from Orychophragmus violaceus was cloned. The full-length cDNA of O. violaceus peroxidase gene (OvRCI, GenBank. Acc. No. AY428037) was 1220 bp and contained an 1128 bp open reading frame encoding a protein of 375 amino acids. Homology analysis and molecular modeling revealed that OvRCI strongly resembled other peroxidase genes. Quantitative real-time PCR analysis revealed that it was a constitutively salt-inducible gene and its transcript level was most abundant after 24 h treatment with 200 mmol.L-1 sodium chloride. Our studies suggested that OvRCI was a new member of the family of recently cloned peroxidase genes.

 

Key words: Orychophragmus violaceus, OvRCI, quantitative real-time PCR.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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