African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines

Irwandi Jaswir1*, Dedi Noviendri1,2, Hamzah Mohd. Salleh1, Muhammad Taher3and Kazuo Miyashita4
1Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Gombak, 53100 Kuala Lumpur, Malaysia. 2Research Center for Marine and Fisheries Product Processing and Biotechnology, Ministry of Marine Affairs and Fisheries, Republic of Indonesia, Petamburan VI, 10260, Central Jakarta, Indonesia. 3Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, 25200 Pahang, Malaysia. 4Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato, Hakodate 041 8611, Japan
Email: [email protected]

  •  Accepted: 07 November 2011
  •  Published: 16 December 2011

Abstract

Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirteen fatty acids were detected with gas chromatography. However, fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents of P. australis were found to be 2.06, 9.50, 6.37, and 2.83%, respectively. For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid with about 23.97%. Furthermore, data obtained from the methyl thiazolyl tetrazolium (MTT) assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value of 2.45 mM.

 

Key words: Brown seaweed, Padina australis, fucoxanthin, fatty acid methyl ester, H1299 cell lines, human lung cancer, MTT assay

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This article is published under the terms of the Creative Commons Attribution License 4.0

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