Two Clariid species, Clarias gariepinus and Heterobranchus bidorsalis were compared at four loci using C. gariepinus microsatellite markers (Cga01, Cga02,Cga03, and Cga05). The heterozygosities observed were found to be 0.450 ± 0.050 and 0.442 ± 0.127, while 100% polymorphism was observed in both species. Overall, 95% of the samples amplified upon polymerase chain reaction (PCR) amplification and 44.3% of the total alleles observed for all the loci were heterozygote. Conformity to Hardy-Weinberg equilibrium using the Chi-Square test showed that 81.25% of locus-population relationship conformed to Hardy-Weinberg equilibrium. The neighbor-joining phylogenetic dendrogram obtained gave a bootstrap value of 72, indicating that the genetic distance between the two species is distinct.
Key words: Microsatellite, Clarias gariepinus, Heterobranchus bidorsalis.
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