African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Preliminary characterization of genetic strains in clariid species, Clarias gariepinus and Heterobranchus bidorsalis using microsatellite markers

  O. T. Agbebi1*, D. E. Ilaboya1 and A. O. Adebambo2        
  1Department of Aquaculture and Fisheries Management, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun state, Nigeria. 2Department of Animal Breeding and Genetics, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun state, Nigeria.
Email: [email protected]

  •  Accepted: 05 October 2012
  •  Published: 23 January 2013

Abstract

 

Two Clariid species, Clarias gariepinus and Heterobranchus bidorsalis were compared at four loci using C. gariepinus microsatellite markers (Cga01, Cga02,Cga03, and Cga05). The heterozygosities observed were found to be 0.450 ± 0.050 and 0.442 ± 0.127, while 100% polymorphism was observed in both species. Overall, 95% of the samples amplified upon polymerase chain reaction (PCR) amplification and 44.3% of the total alleles observed for all the loci were heterozygote. Conformity to Hardy-Weinberg equilibrium using the Chi-Square test showed that 81.25% of locus-population relationship conformed to Hardy-Weinberg equilibrium. The neighbor-joining phylogenetic dendrogram obtained gave a bootstrap value of 72, indicating that the genetic distance between the two species is distinct.

 

 

Key words: Microsatellite, Clarias gariepinus, Heterobranchus bidorsalis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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