African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12145

Full Length Research Paper

Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

Sandra Domingos João Afonso
  • Sandra Domingos João Afonso
  • Federal University of Bahia Recôncavo (UFRB), Rua Rui Barbosa 710, CEP: 44380-000, Cruz das Almas, BA, Brazil.
  • Google Scholar
Ricardo Franco Cunha Moreira
  • Ricardo Franco Cunha Moreira
  • Federal University of Bahia Recôncavo (UFRB), Rua Rui Barbosa 710, CEP: 44380-000, Cruz das Almas, BA, Brazil.
  • Google Scholar
Carlos Alberto da Silva Ledo
  • Carlos Alberto da Silva Ledo
  • Brazilian Agricultural Research Corporation (EMBRAPA Mandioca e Fruticultura), Rua Embrapa S/N CP 007, CEP: 44380-000, Cruz das Almas, BA, Brazil.
  • Google Scholar
Cláudia Fortes Ferreira
  • Cláudia Fortes Ferreira
  • Brazilian Agricultural Research Corporation (EMBRAPA Mandioca e Fruticultura), Rua Embrapa S/N CP 007, CEP: 44380-000, Cruz das Almas, BA, Brazil.
  • Google Scholar
Vanderlei da Silva Santos
  • Vanderlei da Silva Santos
  • Brazilian Agricultural Research Corporation (EMBRAPA Mandioca e Fruticultura), Rua Embrapa S/N CP 007, CEP: 44380-000, Cruz das Almas, BA, Brazil.
  • Google Scholar
Pascoal António Muondo
  • Pascoal António Muondo
  • Agronomic Investigation Institute (IIA), Malanje, Angola.
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  •  Received: 28 November 2018
  •  Accepted: 06 February 2019
  •  Published: 13 February 2019

Abstract

Eighteen ISSR primers (between Simple Repeated Sequences) were used to estimate the genetic diversity of Angolan cassava populations in order to identify genetic variabilities and their potential to be used in the diversification of cassava crops in Angola. In order to do so, three populations from three Angolan provinces were analyzed (Cuanza Norte, Uige and Malanje), which totaled 40 genotypes. In total, 116 bands were amplified with 93.24% polymorphism and 6.4 markers per ISSR primer, on average. Nei’s gene diversity (H) and Shannon indices (I) applied to the analyzed accessions presented values ranging from H = 0.20 to 0.24 and I = 0.29 to 0.50 which are intermediate values. The total gene diversity (HT) was 0.3113, and it showed high heterozygosity in Manihot populations; this outcome indicated that these populations presented gene variability. ANOVA showed 10.41% total variance between populations and 89.59% within them. Molecular featuring revealed gene diversity in each of the assessed populations; there was gene structuring between populations.

 

Key words: Gene diversity, molecular markers, gene flow, polymorphism.