African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Antiproliferative effect of Amburana cearensis seed extracts on human cancer cell lines

Aline Aires Aguiar
  • Aline Aires Aguiar
  • Federal University of Tocantins (UFT), Palmas, Tocantins, Brazil.
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Ernane Gerre Pereira Bastos
  • Ernane Gerre Pereira Bastos
  • Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
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Ilsamar Mendes Soares
  • Ilsamar Mendes Soares
  • Federal University of Tocantins (UFT), Palmas, Tocantins, Brazil.
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Ivana Grivicich
  • Ivana Grivicich
  • Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
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Jéssica Machado Miri
  • Jéssica Machado Miri
  • Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
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Sérgio Donizeti Ascêncio
  • Sérgio Donizeti Ascêncio
  • Federal University of Tocantins (UFT), Palmas, Tocantins, Brazil.
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Raimundo Wagner de Souza Aguiar
  • Raimundo Wagner de Souza Aguiar
  • Federal University of Tocantins (UFT), Palmas, Tocantins, Brazil.
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  •  Received: 19 November 2019
  •  Accepted: 16 January 2020
  •  Published: 31 January 2020

Abstract

Amburana cearensis A. C. Smith (Fabaceae) is a plant of Brazil, popularly known as umburana or cumaru, and is widely used in folk medicine. The population uses its bark and seeds against many pathologies including cancer. The aim of this study was to examine the antiproliferative effect of A. cearensis extracts on human tumor cell lines of HT-29 colon adenocarcinoma and HepG2 hepatocellular carcinoma, as well as on normal mouse fibroblasts L929. This study consists of in vitro tests with extracts obtained from A. cearensis seeds with solvents of increasing polarity against neoplastic and normal cell lines. Knowing that A. cearensis extracts are rich in coumarins and have high antioxidant activity, aspects related to antineoplastic activity and cytotoxicity of four different extracts was evaluated. A. cearensis extracts were analyzed in cancer cells using the MTT assay. Cytotoxicity data demonstrated that methanolic extract (MEA) has activity against HT-29 cell line (IC50 18.8 ± 0.4 µg mL-1) while normal fibroblasts L929 and HepG2 cells were not affected by the extract. The crude seed extracts of A. cearensis did not demonstrate a cytotoxic effect against the cancer cell used in this study; however, the MEA extract can also be promising for this purpose.

 

Key words: Amburana cearensis, cytotoxicity, antiproliferative, tumor cell, coumarin.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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