African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Chemical composition and antioxidant activity of Marrubium vulgare L. essential oil from Tunisia

Adel Kadri1*,  Zied Zarai2 , Ahmed Békir4, Néji Gharsallah3, Mohamed Damak1and Radhouane Gdoura5
1Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, B.P. 1171, 3000 Sfax, University of Sfax, Tunisia. 2Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, BPW, 1173 Sfax, University of Sfax, Tunisia. 3Laboratoire de Biotechnologies Végétales Appliquées à l’Amélioration des Cultures, Faculté des Sciences de Sfax, B.P. 1171, 3000 Sfax. Tunisia. 4Département de Génie des procédés, ISET Sfax, Km 2,5 Rte de Mahdia, 3099 Sfax, University of Sfax, Tunisia. 5Laboratoire de Microbiologie, UR/08-73, Faculté des sciences de Sfax, University of Sfax, Tunisia.
Email: [email protected]

  •  Accepted: 25 April 2011
  •  Published: 09 May 2011

Abstract

In this study, the essential oil of the aerial parts of Marrubium vulgare L. obtained by hydrodistillation was analyzed by gas chromatography coupled to mass spectrometry (GC-MS) in order to determine their chemical composition. Thirty-four(34) components in the oil of M. vulgare were identified. The results demonstrated that the major components of the essential oil were γ-eudesmol (11.93%), β-citronellol (9.90%), citronellyl formate (9.50%) and germacrene D (9.37%). Antioxidant effectiveness was examined by three different methods: The DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, the β-carotene bleaching test and the reducing power assay. The results showed that this oil can be considered an effective source of antioxidants of natural origin. This is the first report on chemical composition of M. vulgare essential oil cultivated in Tunisia and the original study on the antioxidant activity of M. vulgare essential oil.

 

Key words: Marrubium vulgare, essential oil, chemical composition, antioxidant activity.