African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Cloning and functional characterization of a class III chitinase gene from grapevine: Inhibition of fungal growth by recombinant VvChiF III

  S.A. Salami1*, A. Ebadi1, M.R. Naghavi2 and I.B. Dry3
1Department of Horticulture, Faculty of Horticultural Sciences and Plant Protection, University of Tehran, Karaj, Iran.   2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tehran, Karaj 31587, Iran. 3CSIRO Plant Industry, PO Box 350, Glen Osmond SA 5064, Australia.  
Email: [email protected]

  •  Accepted: 20 October 2008
  •  Published: 03 December 2008

Abstract

 

 

To characterize the structure and function of chitinase genes, a class III chitinase gene (VvChiF III) was isolated from Vitis vinifera cv. Flame seedless. The VvChiF III open reading frame comprised 894 nucleotides with no introns and encoded a protein of 297 amino acids. The amino acid sequence encoded by VvChiF III showed a high identity to that of a class III chitinase isolated from V. vinifera cv.  Koshu and to other acidic chitinases. Analysis of the VvChiF III amino acid sequence showed that this gene corresponds to the Glyco-hydro-18 super family that consisting of a signal peptide with the length of 25 amino acids. Purified VvChiF III showed chitinase activity toward the soluble substrate, glycolchitin and antifungal activity against Botrytis cinerea.

 

Key words: Vitis, chitinase genes, recombinant protein, antifungal activity, SNPs.
 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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