African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Coupled biochemical genetic and karyomorphological analyses for taxonomic classification - A case study of Schizothorax species complex (Teleostei: Cyprinidae)

F. A. Ganai*
  • F. A. Ganai*
  • Limnology and Fisheries Laboratory, Centre of Research for Development, Faculty of Biological Sciences, University of Kashmir, Hazratbal Srinagar-190 006. India.
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S. Wani
  • S. Wani
  • Limnology and Fisheries Laboratory, Centre of Research for Development, Faculty of Biological Sciences, University of Kashmir, Hazratbal Srinagar-190 006. India.
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S. Ahmad
  • S. Ahmad
  • Limnology and Fisheries Laboratory, Centre of Research for Development, Faculty of Biological Sciences, University of Kashmir, Hazratbal Srinagar-190 006. India.
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A. R. Yousuf
  • A. R. Yousuf
  • CORD (Zoology), Faculty of Biological Science, University of Kashmir 190006, India.
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N. K. Tripathi
  • N. K. Tripathi
  • Animal Cytogenetics Laboratory, Department of Zoology, School of Biosciences, University of Jammu. India.
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  •  Received: 11 October 2013
  •  Accepted: 24 March 2014
  •  Published: 09 April 2014

Abstract

Biochemical genetic and karyomorphological studies were evaluated for taxonomic importance. Five Schizothorax species namely: Schizothorax niger, Schizothorax curvifrons, Schizothorax esocinus, Schizothorax labiatus and Schizothorax plagiostomus were studied in this experiment as a model for serum proteins and chromosomal analysis. Serum protein band patterns of Schizothorax species complex were studied by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Their electrophoretograms revealed similarities as well as differences in the number and molecular weight of protein bands. S. esocinus showed five bands, S. curvifrons five, S. niger seven, S. labiatus and S. plagiostomus each showed six bands; they also showed species characteristic bands. Karyotypic study of these was carried out. The diploid chromosome numbers recorded were 98 in S. niger (24 m + 32 sm + 22 st + 20 t), 98 in S. esocinus (30 m + 22 sm + 10 st + 36 t), 98 in S. labiatus (24 m + 20 sm + 2 st + 52 t), 96 in S. plagiostomus (24 m + 18 sm + 5 4t) and 94 in S. curvifrons (26 m + 20 sm + 20 st + 28 t). Coupled biochemical genetic and karyomorphological analysis proved a good taxonomic tool as the results were decisive in establishing the species status of these species despite their overlapping morphological characters.

 

Key words: Schizothorax, serum proteins, karyotype, taxonomy, biochemical.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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