African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Assessment of biodiversity based on morphological characteristics and RAPD markers among genotypes of wild rose species

Atif Riaz1,2*, Mansoor Hameed3, Azeem Iqbal Khan4, Adnan Younis1 and Faisal Saeed Awan4
1Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan. 2Plant Breeding Institute, Faculty of Food and Agriculture, University of Sydney, Australia. 3Department of Botany, University of Agriculture, Faisalabad, Pakistan. 4Centre of Agricultural Biochemistry and Biotechnology (CABB), University ofAgriculture Faisalabad, Pakistan.
Email: [email protected]

  •  Accepted: 01 July 2011
  •  Published: 03 October 2011

Abstract

Conservation and utilization of the native plant resources is essential for long termsustainability of biodiversity. Wild native resources are adapted to specific and diverse environmental conditions and therefore, these adaptive features can be introduced into modern cultivars either through conventional breeding or advanced molecular genetic techniques. Understanding the genetic make up of the wildly growing plant species and of target desirable genes is a prerequisite for this purpose. Five wild rose (Rosa L.) genotypes were collected from different locations in northern hilly areas of Pakistan for this study. Different morphological characteristics and PCR based random amplified polymorphic DNA (RAPD) technique was used to find out the diversity and relationship among the genotypes.On morphological basis, Rosa webbiana collected from Muree and Nathia gali showed maximum (83%) similarity, whereas on DNA pattern basis, Rosa brunoniicollected from Bansra gali and Sunny bank showed maximum (72%) similarity, while R. webbiana showed maximum diversity among all the species.

 

Key words: Genetic diversity, morphological differences, random amplified polymorphic DNA (RAPD), Rosa.