African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Polymorphisms in 5’-upstream region of the myostatin gene in four chicken breeds and its relationship with growth traits in the Bian chicken

Zhang Genxi1, 3, Dai Guojun1, 3 , Wang Jinyu1,3, *, Wei yue1,3, Ding Fuxiang2, Li Zhang2, Zhao Xiuhua1,3, Xie Kaizhou 1,3,  Wang wenhao1,3
  1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. 2Institute of Animal Husbandry and Veterinary, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China. 3Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu  province, Yangzhou 225009, China
Email: [email protected]

  •  Accepted: 11 May 2012
  •  Published: 17 May 2012

Abstract

 

Myostatin is a member of the transforming growth factor-β family with a key role in inhibition of muscle growth by negative regulation of both myoblast proliferation and differentiation. Single nucleotide polymorphisms (SNPs) of the 5’-upstream region of the myostatin gene were detected by single-stranded conformation polymorphism (SSCP) and DNA sequencing in the Bian, Jinghai, Youxi and Arbor Acre chickens. Four novel mutations (G673A, G985C, G1085A, and A1278T) were detected. Only one locus (A1278T) displayed polymorphism in the Bian chicken, and thus, three genotypes (QQ, QR, and RR) were formed. Association analysis showed that the Bian chicken of the RR and QR genotypes had significantly higher body weight than those of the QQ genotype (P < 0.05 or P < 0.01) from 14 to 18 weeks of age. The novel mutation (A1278T) could be a useful molecular marker for planning improvements in the growth traits of the Bian chicken.

 

Key words: Bian chicken, myostatin gene, polymorphism, growth traits.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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