African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

The isolation and characterization of twelve novel microsatellite loci from Haliotis ovina

Zhongbao Li*, Xinjiang Tian, Yuanyu Cao, Guiling Zhang, Zhihong Zhang, Lina Chen and Xiaojiao Chen
Fisheries College, Institute of Aquaculture Biotechnology, Jimei University, Xiamen, China.
Email: [email protected]

  •  Accepted: 05 July 2012
  •  Published: 18 December 2013

Abstract

Twelve (12) microsatellite loci were developed from Haliotis ovina by magnetic bead hybridization method. Genetic variability was assessed using 30 individuals from three wild populations. The number of alleles per locus was from 2 to 5 and polymorphism information content was from 0.1228 to 0.6542. The observed and expected heterozygosities ranged from 0.0000 to 0.7778 and 0.1288 to 0.6310, respectively. These loci should provide useful information for genetic studies such as genetic diversity, pedigree analysis, construction of genetic linkage maps and marker-assisted selection breeding in H. ovina.

 

Key words: Genetic markers, Haliotis ovina, microsatellites.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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