African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

Full Length Research Paper

Lipase-producing fungi for potential wastewater treatment and bioenergy production

Celson Rodrigues1*, Sérvio Túlio Alves Cassini1, Paulo Wagner Pereira Antunes1, Laura Marina Pinotti2, Regina de Pinho Keller1 and Ricardo Franci Gonçalves1
1Technological Center, Department of Environmental Engineering, Federal University of Espírito Santo-UFES, Campus Goiabeiras, Avenue Fernando Ferrari 514, CEP 29075-910, Vitória - ES, Brazil. 2Department of Engineering and Technology, University Center North of Espírito Santo, Federal University of Espírito Santo - UFES, Campus of São Mateus, Highway BR 101 North Km 60, CEP 29932-540, São Mateus - ES, Brazil.
Email: [email protected]

  •  Received: 23 April 2015
  •  Accepted: 08 April 2016
  •  Published: 04 May 2016


The use of fungal biomass as a lipase biocatalyst represents an attractive approach for the treatments of oil wastewater as well as for the production of biodiesel from oil and residual grease, due to its greater stability, possibility of reuse, and lower cost. In this work, 20 filamentous fungi were isolated from the grease trap scum of a restaurant at the Federal University of Espírito Santo, Brazil. The fungi included those belonging to the genera Aspergillus, Beauveria, Botrytis, Cladosporium, Colletotrichum, Fusarium, Geotrichum, Penicillium, Rhizomucor, and Verticillium. Fungal lipase activity and biomass production were quantified. Lipase activity ranged from 0.13 U mg-1 protein of Rhizomucor sp. ECGF18 to 18.06 U mg-1 protein of Penicillium sp. ECGF02, and the biomass production ranged from 7.61 mg mL-1 for Cladosporium sp. ECGF19 to 12.68 mg mL-1 for Rhizomucor sp. ECGF18. In the sequence, Penicillium sp. ECGF02 and Rhizomucor sp. ECGF18, were previously select and, further evaluated in solid-state fermentation. Results confirmed the high extracellular lipase-activity of Penicillium sp. ECG02 and the high intracellular lipase activity of Rhizomucor sp. ECG18. Rhizomucor sp. ECG18 showed potential for use in future research, in the form of whole-cell lipases, wastewater treatment, and as a biocatalyst in the production of biodiesel from oil residues.


Key words: Lipase-producing fungi, wastewater treatment, bioenergy.