African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Cellulase production by Trichoderma sp. on apple pomace under solid state fermentation

Haiyan Sun1, Xiangyang Ge2, Zhikui Hao2 and Ming Peng1*
  1Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. 2The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, China.
Email: [email protected]

  •  Accepted: 04 June 2009
  •  Published: 11 January 2010

Abstract

 

The feasibility of using apple pomace for cellulase production by Trichoderma sp. under solid state fermentation was evaluated in this study. Our results indicated that initial moisture level of the medium, incubation temperature and inoculum size influenced the cellulase production greatly. The optimum initial moisture level, incubation temperature and inoculum size were 70%, 32 and 2×10spores/flask, respectively. The supplement of lactose and corn-steep solid to the apple pomace favored the enzyme formation markedly.

 

Key words: Apple pomace, cellulase, Trichoderma sp.