African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12267

Full Length Research Paper

Carotenoids from Phaffia rhodozyma: Antioxidant activity and stability of extracts

Eliane Pereira Cipolatti*
  • Eliane Pereira Cipolatti*
  • Bioprocess Engineering Laboratory, School of Chemistry and Food, Federal University of Rio Grande (FURG), Av. Italia, Km 8, 96203-900, Rio Grande, RS, Brazil.
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Bruna Araujo Bulsing
  • Bruna Araujo Bulsing
  • Bioprocess Engineering Laboratory, School of Chemistry and Food, Federal University of Rio Grande (FURG), Av. Italia, Km 8, 96203-900, Rio Grande, RS, Brazil.
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Carolina dos Santos Sa
  • Carolina dos Santos Sa
  • Bioprocess Engineering Laboratory, School of Chemistry and Food, Federal University of Rio Grande (FURG), Av. Italia, Km 8, 96203-900, Rio Grande, RS, Brazil.
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Carlos Andre Veiga Burkert
  • Carlos Andre Veiga Burkert
  • Bioprocess Engineering Laboratory, School of Chemistry and Food, Federal University of Rio Grande (FURG), Av. Italia, Km 8, 96203-900, Rio Grande, RS, Brazil.
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Eliana Badiale Furlong
  • Eliana Badiale Furlong
  • Food Science Laboratory, School of Chemistry and Food, Federal University of Rio Grande (FURG), Av. Italia, Km 8, 96203-900, Rio Grande, RS, Brazil.
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Janaina Fernandes De Medeiros Burkert
  • Janaina Fernandes De Medeiros Burkert
  • Bioprocess Engineering Laboratory, School of Chemistry and Food, Federal University of Rio Grande (FURG), Av. Italia, Km 8, 96203-900, Rio Grande, RS, Brazil.
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  •  Received: 29 April 2015
  •  Accepted: 08 June 2015
  •  Published: 10 June 2015

Abstract

The main goal of this work was to establish the stability and antioxidant activity of the extracts obtained through different techniques for recovering carotenoids from Phaffia rhodozyma NRRL-Y 17268. The best conditions for extracting carotenoids through cell rupture with dimethylsulfoxide (DMSO) were found to be a particle size of 0.125 mm submitted to freezing temperature (-18°C) for 48 h (272 µg/g). For DMSO extracts, freezing negatively affected the antioxidant activity by 2,2 '-azinobis (3-ethyl benzothiazoline-6-sulfonic acid)) and DPPH (2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. The carotenogenic extracts obtained by enzymatic disruption proved to be more promising in relation to its antioxidant activity.

 

Key words: Microbial carotenoids, antioxidant properties, cell wall disruption.