African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Analysis of genetic variability in rice cultivars of Arunachal Pradesh (India) using microsatellite marker

S. R. Berilus1, A. Pattanayak2 and Gurudayal Ram3*
  1University of Science and Technology, Meghalaya (USTM), Ribhoi, Meghalaya, India. 2Division of Plant Breeding, ICAR Research Complex of NEH Region, Umiam, Meghalaya India. 3Jacob School of Biotechnology and Bioengineering, SHIATS, Allahabad, Uttar Pradesh, India.
Email: [email protected]

  •  Accepted: 31 October 2012
  •  Published: 20 February 2013

Abstract

 

The present investigation was carried out to analyze the genetic diversity of rice cultivars utilizing microsatellite or simple sequence repeat (SSR) markers. Twenty four (24) genotypes of rice were evaluated for polymorphisms after amplification with 30 SSR primer pairs. The results obtained depicted a wide range of variation among the varieties. The diversity parameters confirmed that Bordungsha F1, S5 and Baodhan Bordungsha (deep water rice) were most diverse and were distinctly different from the rests of the genotype and the dissimilarity coefficient ranged from 0.06 to 0.94. This study provides a clear picture of the genetic diversity of rice. Among the set of SSR primers tested, RM44 (0.942), RM495 (0.932), RM125 (0.998) and OSR13 (0.970) were more informative in comparison to other primers.

 

Key words: Oryza sativa, SSR markers, PCR, polymorphism, genetic variation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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