African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Use of simple sequence repeat (SSR) markers for screening blue disease resistance in cotton germplasm exchange

Christopher Faustine
  • Christopher Faustine
  • Department of Life Sciences, The Open University of Tanzania, P.O. Box 23409 Dar es salaam Tanzania.
  • Search for this author on:
  • Google Scholar
Lucia Vieira Hoffmann
  • Lucia Vieira Hoffmann
  • Embrapa, Rodovia GO-462, km 12, Rural Zone, Santo Antonio de Goias, 75375-000, Brazil.
  • Search for this author on:
  • Google Scholar
Flora Ismail Tibazarwa*
  • Flora Ismail Tibazarwa*
  • Tanzania Commission for Science and Technology, Dar es Salaam, P.O Box 4302, Tanzania.
  • Search for this author on:
  • Google Scholar
Everina Lukonge
  • Everina Lukonge
  • Ukiriguru Agricultural Research Institute, P. O. Box 1433 Mwanza, Tanzania.
  • Search for this author on:
  • Google Scholar


  •  Received: 28 May 2015
  •  Accepted: 13 August 2015
  •  Published: 14 October 2015

Abstract

Blue disease of cotton is an economically important disease of the crop first described from the Central African Republic and spread to other countries. Brazil and other South American countries record crop losses of up to 80% from infection but no cases of the disease have been reported in Tanzania. Resistance to the disease has been found in African germplasm and transferred to crop cultivars worldwide. Molecular markers linked to blue disease resistance genes have been identified presenting useful tools to identify resistant germplasm. All plants of three Tanzanian cotton cultivars (Gossypium hirsutum L.) UK91, UK08 and UKM08 showed resistance alleles for both the simple sequence repeat (SSR) (DC20027-202 bp) and single nucleotide polymorphisms (SNP) (NG0204310-C) markers but some plants of the Brazilian cultivars (G. hirsutum L.); Ipê, Cedro, Aroeira and Araça lacked resistance alleles. The findings suggest the need for caution to be taken during introduction of exotic germplasm and recognize the value of resistance trait to susceptible Brazilian germplasm when breeding for blue disease resistance.
 
Key words: Cotton blue disease, cotton single nucleotide polymorphisms (SNPs), simple sequence repeat (SSR), resistant alleles in cotton.
 

Abbreviation

SSR, simple sequence repeat; CBD, Cotton blue disease; ECGA, eastern cotton growing area; BYDV, barley yellow dwarf virus; MAS, marker assisted selection; WCGA, western cotton growing area.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 14 No. 41
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES