Abstract

The analysis of leaf and in vitro calli extracts of Tylophora indica indicated qualitative difference in the phytochemical compounds. The minimum inhibitory concentration (MIC) of selected solvent extracts of the leaf ranged from 20 to 80 mg/ml. Ethanolic and methanolic extracts were effective in inhibiting most of the tested pathogens but the aqueous extract had poor inhibition. Both the leaf and calli extracts had varying inhibitory action against the test organisms. It may be concluded that in vitro calli culture methods could lead to maintain bioactive potential of plants and to formulate antimicrobial drugs of natural origin.

Key words: Tylophora indica, leaf, calli, phytocompounds, agar diffusion, pathogens,in vitro culture.