African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12364

Full Length Research Paper

Genetic diversity of Pyrenophora tritici-repentis in Algeria as revealed by amplified fragement length polymorphism (AFLP) analysis

  Hamida Benslimane1*, Samer Lababidi2, Amor Yahyaoui3, Francis Ogbonnaya2, Zouaoui Bouznad4 and Micheal Baum2  
  1Université M’hamed Bougra, Boumerdès, Faculté des Sciences, Département de Biologie, Laboratoire de Biologie Moléculaire, 16 Avenue Rue de l’indépendance, Boumerdès, Algérie. 2International Center for Agricultural Research in Dry Areas, P.O. Box 5466, Aleppo Syria. 3International Maize and Wheat Improvement Center, Apdo. Postal 6-641,06600, Mexico DF, Mexico. 4Ecole Nationale Supérieure d’Agronomie, Département de Botanique, El-Harrach, Alger, Algérie.  
Email: [email protected]

  •  Accepted: 25 June 2013
  •  Published: 26 June 2013

Abstract

 

Tan spot caused by Pyrenophora tritici-repentis is a major wheat disease. DNA of 61 isolates of P. tritici-repentis from different cereal growing areas in Algeria were analyzed using amplified fragment length polymorphism (AFLP) in order to  study the genetic diversity among this population. Initially, 78 primer combinations were tested, of which 12 were selected and applied to the 61 isolates. There was a high genetic diversity in this population of P. tritici-repentis with 61 different haplotypes among the 61 isolates selected. The Jaccard similarity index range was 1.43 to 68.37%. Cluster analysis showed that, clustering of isolates was independent of their race classification, geographic origin, or host plant. However, one isolate (Ptr24) that showed a new virulence pattern in our previous race analysis study was clearly distinguished from the rest of the population studied. This isolate had not only new virulence but also different genetic makeup to other P. tritici-repentis isolates and requires additional studies to decipher complete knowledge of host-pathogen interactions for tan spot of wheat.

 

Key words: Pyrenophora tritici-repentis, genetic diversity, AFLP, Algeria.