African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Free radical scavenging activity of Pterogyne nitens Tul. (Fabaceae)

  Luis Octávio Regasini1*, Camila Martins de Oliveira1, José Carlos Rebuglio Vellosa2, Olga Maria Mascarenhas de Faria Oliveira2, Dulce Helena Siqueira Silva1 and Vanderlan da Silva Bolzani1
1Department of Organic Chemistry, Institute of Chemistry, São Paulo State University, Araraquara, Brazil.   2Department of Biochemistry and Technological Chemistry, Institute of Chemistry, São Paulo State University, Araraquara, Brazil.  
Email: [email protected]

  •  Accepted: 30 October 2008
  •  Published: 17 December 2008

Abstract

 

 

As part of our ongoing research on antioxidant agents from Brazilian flora, twenty extracts and fractions obtained from Pterogyne nitens Tulasne (Fabaceae) were screened for free radical scavenging activity by using ABTS [2,2’-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)] and DPPH (2,2-diphenyl-1-picrylhydrazyl-hydrate) radicals colorimetric assay and β-carotene bleaching test. The strongest activity was found in ethyl acetate fraction from the stem barks, exhibiting IC50 values (in µg/ml) of 2.10 ± 0.1 and 10.2 ± 0.3 on ABTS•+ and DPPH•, respectively. Additionally, chromatographic fractionation of stem barks yielding myricetin, quercitrin and mirycetrin, three flavonols with remarkable antioxidant activity.

 

Key words: Antioxidant, free radical scavenging activity, DPPH, ABTS, Pterogyne nitens, Leguminosae, Fabaceae, Caesalpinioideae, flavonoid, flavonol.
 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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