African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Characterization of pseudorabies viruses produced in mammalian cells by viral genome transfection

Yudong Ren1,2, Guangxing Li1, Dingding Su3, Qing Yang4* and Xiaofeng Ren1*    
1College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China. 2College of Engineering, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China. 3Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Grop , Hunan Agricultural University, 1 Nongda Road, Furong Disctrict, Changsha 410128, China. 4College of Veterinary Medicine, Hunan Agricultural University, 1 Nongda Road, Furong Disctrict, Changsha 410128, China.    
Email: [email protected] or [email protected]

  •  Accepted: 29 June 2010
  •  Published: 28 February 2012

Abstract

The full-length genome DNA of pseudorabies virus (PrV) was transfected into swine testis (ST) cells using calcium phosphate method to generate the infectious viruses. The resulting viruses were identified by indirect immunofluorescence and were used to infect African green monkey kidney (Vero) cells. Our results showed that the produced viruses had similar plaque morphology and growth properties with PrV vaccine strain, Bartha-K61. This method may produce PrV at an inexpensive cost.

 

Key words: Pseudorabies virus, transfection, virus plaque, cytopathic effect.
 

 

Abbreviation

 

 PrV, Pseudorabies virus; ST, swine testis; HSV-1, herpes simplex type 1 virus; AD, Aujeszky's diseases; EMEM, Eagles’ minimal essential medium;DMEM, Dulbecco´s modified Eagle's medium; NBS, newborn bovine serum; PBS,phosphate-buffered saline; BBS, borate buffered saline; MOI, multiplicity of infection;p.i, post infection; CPE, cytopathic effect; PEDV, porcine epidemic diarrhea virus

 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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