African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12504

Full Length Research Paper

Establishment of an efficient regeneration protocol of six different varieties of wheat (Triticum aestivum L.)

Bo Chen
  • Bo Chen
  • School of Agriculture, Forestry and Food Engineering, Yibin University, 644000 Yibin, Sichuan, China.
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Muhammad Tahir
  • Muhammad Tahir
  • Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100091 Beijing, China.
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Fatima
  • Fatima
  • Department of Mathematics, University of Karachi, 75270 Karachi, Sindh, Pakistan.
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Sara Zafar
  • Sara Zafar
  • Botany Department, Government College University, 38000 Faisalabad, Punjab, Pakistan.
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Hui Li
  • Hui Li
  • College of Forestry, Inner Mongolia Agricultural University, 010019 Hohhot, China.
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Jia Li
  • Jia Li
  • School of Agriculture, Forestry and Food Engineering, Yibin University, 644000 Yibin, Sichuan, China.
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Arthur J. Ragauskas
  • Arthur J. Ragauskas
  • Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee Institute of Agriculture, Knoxville, TN 37996, USA.
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Caiming Gou
  • Caiming Gou
  • School of Agriculture, Forestry and Food Engineering, Yibin University, 644000 Yibin, Sichuan, China
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Hayssam M. Ali
  • Hayssam M. Ali
  • Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
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Manzar Abbas
  • Manzar Abbas
  • School of Agriculture, Forestry and Food Engineering, Yibin University, 644000 Yibin, Sichuan, China.
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  •  Received: 11 December 2023
  •  Accepted: 25 January 2024
  •  Published: 29 February 2024

Abstract

An efficient callogenesis and regeneration protocol was optimized for six different wheat (Triticum aestivum L.) varieties including Chakwal-50, Galaxy-01, NARC-09, Pakistan-13, Millat-11, and Borlog-14. For callogenesis, mature seeds of each variety were used as explant, surface sterilization, and culturing were carried out on MS medium enhanced with dissimilar levels (0.5, 1.5, 2.5, 3.5, 4.5, and 5.5 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D). For regeneration, six different treatments with different combinations of IAA (1.5, 3, and 4.5 mg/L) and Kinetin (1, 2, 3, and 4 mg/L) were applied on each variety. Highest frequency of callogenesis (41.66%) was attained on MS medium in addition with 2.5, 5.5 and 3.5 mg/L 2,4-D in Millet-11, followed by Chakwal-50 (33.33%) and Borlog-14 (29.16%), respectively. Maximum regeneration (41.66%) was observed in Millat-11 on medium enhanced with 1.5 mg/L IAA and 2 mg/L Kinetin. Among all, Millet-11 appeared most responsive genotype towards callogenesis and regeneration, while NARC-09 (16.66%) was least responsive. This study will open new avenues for biofortification of Mellet-11 wheat variety and improvement against biotic and abiotic stress.

 

Key words: Wheat, tissue culture, callogenesis, regeneration, Millet-11, 2,4-D Kinetin.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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