African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

In vitro pollen quantity, viability and germination tests in quince

Zeynel Dalkiliç1* and H. Osman Mestav1,2
1Department of Horticulture, Faculty of Agriculture, Adnan Menderes University, South Campus, 09100 Aydin, Turkey. 2Çine Vocational School, Adnan Menderes University, 09500 Aydin, Turkey.
Email: [email protected]

  •  Accepted: 12 October 2011
  •  Published: 21 November 2011

Abstract

Quince (Cydonia oblonga Mill.) cvs. Ekmek, EÅŸme, Limon, Ege 2, Ege 22, Ege 25 and Quince-A rootstock pollens were collected in April from the unopened pink balloon-stage flowers on mature trees. The pollen amount was between 20063 pollen/flower (‘Ege 25’) and 11906 pollen/flower (‘Limon’) with hemacytometer. The lowest and highest pollen viability were determined from 79.8 (Quince A in day 14) to 97.8% (‘Ege 2’ in day 0) by IKI. The germination rates in different sucrose and boric acid concentrations in 1% agar changed from 0 (‘Limon’ in 0% sucrose) to 91.7% (‘Ege 2’ in 20% sucrose) and from 32.7 (Quince A in 0 mg/L boric acid) to 94.9% (‘Ege 22’ in 100 mg/L boric acid), respectively.

 

Key words: Cydonia oblonga, pollen, viability, germination.