African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

Short Communication

Characterization of potential ethylene-producing rhizosphere bacteria of Striga-infested maize and sorghum

Olubukola O. Babalola1,2*, Elie O. Osir2  and Abiodun I. Sanni1
  1Department of Botany and Microbiology, University of Ibadan, Ibadan Nigeria 2International Centre of Insect Physiology and Ecology, Nairobi, Kenya
Email: 234-803-703-5965

  •  Accepted: 12 November 2002
  •  Published: 30 December 2002

Abstract

 

Three rhizosphere bacteria, Pseudomonas sp., Enterobacter sakazakii andKlebsiella oxytoca, were analyzed for genetic variation. DNA fingerprint patterns of the three bacteria were markedly different when amplified with different primers. In total, 68 bands were produced by the three primers, 62 of which where variable. The number of polymorphic RAPD loci per isolate ranged from one to 13. Cluster analysis indicated that E. sakazakii  and K. oxytoca are the most closely related of the three.

 

Key words: Bacteria, RAPD-PCR, Striga hermonthica.