African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Bioassay-based screening of myxobacteria producing antitumor secondary metabolites

Wen-Jie Guo1,3, Feng-Jie Cui1 and Wen-Yi Tao1,2*
    1School of Biotechnology, Southern Yangtze University, Wuxi, P.R. China, 214122.         2The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University.China.   3Gujing Group, Bozhou, Anhui Province, P.R.China, 236820.
Email: [email protected]

  •  Accepted: 12 November 2007
  •  Published: 30 April 2008

Abstract

 

Myxobacteria are gliding gram-negative bacteria and a class of prokaryote with complicated multicellular behaviors and morphogenesis.  Reports show that myxobacteria generally produce large families of secondary metabolites with various bioactivities, such as antifungal and anti-tumor activities.  In this paper, two strains producing metabolites with high anticancer bioactivity to 5 tumor cell lines were screened from over 370 isolated samples by adopting cell culturing technology and cell toxicity tests. The strains were identified as genuspolyangium (Polyangium vitellinum and Sorangium cellulosum), named JSW103 and AHB125, based on the morphology of vegetative swarms, fruiting bodies, vegetative cells and myxospores.   IC50 values of JSW103 to tumor cell lines B16 and SGC7901 were 0.2715 μg ml-1 and 4.1924 μg ml-1, respectively, while for AHB125 metabolites to tumor cell lines B16 and SGC7901, the values were 0.0082 μg ml-1 and 0.036 μg  ml-1, respectively.

 

Key words: Antitumor activity, bioassay-based screening, IC50, myxobacteria, secondary metabolites.