African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

A novel dynamic perfusion system for stem cell proliferation and timely observation

Shanglong Xu1,3*, Dichen Li2, Junjun Lu3, Mengdong Lei1 and Wei Li3
1Department of Mechatronics Engineering, University of Electronic Science and Technology of China, Chengdu, China. 2State Key laboratory of Mechanical Manufacture System Engineering, Xi’an Jiaotong University, Xi’an, China. 3State Key Laboratory of Oral diseases, Sichuan University, Chendu, China
Email: [email protected]

  •  Accepted: 04 November 2011
  •  Published: 14 December 2011

Abstract

A novel dynamic perfusion system has been developed to provide fluid flow and mass transport for cell proliferation inside a scaffold. It was mainly composed of a micro-device, a peristaltic pump and two silicone tubes. The material of the device was polymethyl methacrylate, which is biocompatible and penetrates. The mesenchymal stem cells were labeled with DiI for 14 days’ culture in the micro-device. It was shown from the experiment of dynamic culture that cells were well adhered to the scaffolds and cell growth could be observed directly and timely. Cell distribution within the scaffold was uneven for different nutrients supply and fluid shear stresses. The designed micro-device allows the continuous and dynamic observation in a large view during the culture of cells and could be used to study the effect of fluid shear stresses on cell proliferation in a direct view.

 

Key words:  Perfusion system, scaffolds, cell, flow channel, observation.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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