African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Recovery of DNA from agarose gel by trap method

Hongli Xia1, Zui Tan1*, Jianguo Qiao2 and Chuan Liang1
  1Department of Vascular Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. 2Department of General Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Email: [email protected]

  •  Accepted: 10 May 2011
  •  Published: 30 September 2011

Abstract

 

Recovery of DNA from agarose gel electrophoresis is a basic operation during molecular cloning. Circular or linear DNA fragments which vary from 1.5 to 6.5 kb and correspond to 1 kb marker can be recovered from 0.8 to 1.0% agarose gel smoothly with a simple and rapid trap method. The recovery efficiency could be more than 70% and the quality of the recovered DNA is proved to be good enough for future research. Here, we provided another good method to recover DNA from agarose gel besides using commercial kits.

 

Key words: Trap method, recovery of DNA, agarose gel electrophoresis.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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