Abstract

In the present investigation, binary combination of photo activated cow urine was determined against seven bacterial strains. Photoactivated cow urine showed MIC value of 0.25 ml/ml against Staphylococcus aureus (ATCC 25923), Bacillus cereus(ATCC 11778), Lactobacillus acidophilus (ATCC 53103 and Micrococcus luteus(ATCC 9341), while it had 0.125 ml/ml against Escherichia coli (ATCC 25922). Binary combinations of cow urine with Neem and Bavchi oil showed synergistic effect as the MIC value obtained was between 0.125 - 0.25 ml/ml. Similarly, photoactivated cow urine showed least MBC value of 0.25 - 1.0 ml/ml. Furthermore, growth inhibition zone diameters obtained in the presence of photoactivated cow urine and its binary combinations were found much larger than antibiotic drugs. Neem oil showed highest inhibition zone diameter of 45 mm against Streptococcus pneumoniae. Neem oil and cow urine showed 33 - 35 mm inhibition zone diameter against B. cereus, L. acidophilus, Micrococcus luteus, Klebsiella pneumoniae and S. pneumoniae, while Bawchi oil and cow urine combination showed a higher inhibition zone diameter of 41 mm against K. pneumoniae. Photoactivated cow urine showed 32 - 36 mm inhibition zone diameter homogeneously against all bacterial strains. It proved very high antimicrobial susceptibility of cow urine and essential oils.

Key words: Antimicrobial activity, cow urine and essential oil.