Full Length Research Paper
Abstract
A soil bacterium isolated from a contaminated site degraded phenol as the sole carbon and energy source was identified as Xanthobacter flavus MTCC 9130. This microbial strain was able to tolerate phenol up to 1100 mg/l concentration. The lag phase increased with the increase in phenol concentration. The optimum growth temperature was 37°C. The organism could degrade completely within 120 h when initial concentration was less than 600 mg/l. Enzyme assay through cell free extract showed the presence of catechol-1,2-dioxygenase. The specific activity was 0.146 µmol/min/mg protein. However higher concentrations of phenol in the medium showed a negative effect on the growth of the bacterium. Hence X. flavus can be effectively used for bioremediation of phenol-contaminated sites.
Key words: Bioremediation, catechol-1, 2-dioxygenase, ortho-pathway, phenol,Xanthobacter flavus.
Abbreviation
Abbreviations: MSM, mineral salts medium; IMTECH, Institute of Microbial Technology; MTCC, Microbial Type Culture collection; PAH’s, Polyaromatic hydrocarbons, HAC’s haloaromatic compounds.
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