African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Identification of a latent pathogen on mulberry tree with a disease of mosaic dwarf

Jianming Fei1, Yufeng Li1, Yan Wu2, Xichuang Bai3, Guofang Shi1, Feng Yu2 and Yuanzhang Kuai4*
  1Huzhou Academy of Agricultural Sciences, Huzhou 313000, China. 2Institute of Life Science, Jiangsu University, Zhenjiang 212013, China. 3Wuxing Station of Silkworm and Mulberry technical Guidance, Huzhou 31300, China. 4Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212008, China.
Email: [email protected]

  •  Accepted: 01 September 2009
  •  Published: 19 October 2009

Abstract

 

A disease on mulberries with the typical symptoms of mosaic and dwarf leaves was found in middle areas of China in 1980s. Presently, this disease became serious and spread out. Based on previous finding, we detected a viroid-like small molecular RNA in diseased mulberries tissues. In this paper, we further identified the pathogen of mulberry mosaic dwarf disease (MMDD) according to the Koch's postulates and reported the diagnostic method of the pathogen by using PCR with two sets of specific primers. The result might be helpful to control the disease extension.

 

Key words: Mulberry, mosaic dwarf disease, viroid-like RNA, identification.