African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Comparison of Luffa cylindrica L. sponge discs and Ca-alginate gel beads as immobilized matrices of Saccharomyces cerevisiae for bio-ethanol production from sugarcane molasses

Shuvashish Behera1 and Ramesh C. Ray2*
  1Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, 751004, Odisha, India.   2Microbiology Laboratory, Central Tuber Crops Research Institute (Regional Centre), Bhubaneswar, 751019, Orissa, India.
Email: [email protected]

  •  Accepted: 10 May 2012
  •  Published: 27 November 2012

Abstract

 

The production of bio-ethanol from cane molasses (diluted to 15% sugar, w/v) was studied, using the yeast, Saccharomyces cerevisiae immobilized in luffa (Luffa cylindrica L.) sponge discs and in Ca-alginate gel beads. At the end of 96 h fermentation, the ethanol yields were 64.67 ± 0.016 and 65.21 ± 0.030 g/l molasses, with luffa and Ca-alginate entrapped S. cerevisiae cells exhibiting 89.90 ± 0.008 and 91.86 ± 0.072% sugar conversion, respectively. There was no statistically significant difference [Fisher’s least significance difference (LSD)] between the two immobilized systems in both sugar utilization (t = 0.254, p < 0.801) and in ethanol production (t = -0.663, p < 0.513).
 
Key words: Bio-ethanol, cell immobilization, fermentation, molasses,Saccharomyces cerevisiae

Abbreviation

MYGP, Malt extract-yeast extract-glucose-peptone; ANOVA, analysis of variance; LSD, least significance difference.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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