African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Full Length Research Paper

Evaluation of genetic diversity of okra accessions [Abelmoschus esculentus (L. Moench)] cultivated in Burkina Faso using microsatellite markers

Mahamadi Hamed OUEDRAOGO
  • Mahamadi Hamed OUEDRAOGO
  • Laboratoire Biosciences, Université Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Nerbewende SAWADOGO
  • Nerbewende SAWADOGO
  • Laboratoire Biosciences, Université Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Teyoure Benoit Joseph BATIENO
  • Teyoure Benoit Joseph BATIENO
  • Laboratoire de Génétique et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), CREAF de Kamboinsé (Burkina Faso) 04 BP 8645 Ouagadougou, Burkina Faso.
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Wend-Pagnangde Marie Serge Felicien ZIDA
  • Wend-Pagnangde Marie Serge Felicien ZIDA
  • Laboratoire de Génétique et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), CREAF de Kamboinsé (Burkina Faso) 04 BP 8645 Ouagadougou, Burkina Faso.
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Ali Lardia BOUGMA
  • Ali Lardia BOUGMA
  • Laboratoire Biosciences, Université Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Antoine BARRO
  • Antoine BARRO
  • Laboratoire Biosciences, Université Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Zakaria KIEBRE
  • Zakaria KIEBRE
  • Laboratoire Biosciences, Université Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Mahamadou SAWADOGO
  • Mahamadou SAWADOGO
  • Laboratoire Biosciences, Université Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 20 November 2017
  •  Accepted: 19 January 2018
  •  Published: 31 January 2018

Abstract


Okra is a traditional vegetable grown throughout Burkina Faso. Despite a food and non-food valorization of all parts of the plant, its genetic diversity is still little known. Thus, 50 accessions of okra from Burkina Faso were characterized using 19 microsatellite markers in order to determine the level and structure of genetic diversity. The results reveal a total of 34 alleles including 3 rare alleles and a number of 2.58 effective alleles. A polymorphic information content (PIC) value between 0.11 and 0.86 and markers polymorphism rate of 42.10% were also obtained. Mean expected heterozygosity and Shannon diversity index were 0.46 and 0.77, respectively. In addition, a structuring of the 50 accessions in three genetic groups with indices of very similar accessions of 88 to 95% between climatic zones and 83 to 95% between ethnic groups were observed. The diversity obtained could be exploited in the program of selection and varietal improvement of okra.

 
Key words: Genetic variability, simple sequence repeats (SSR) markers, varietal selection, valorization, genetic differentiation.