African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12291

Full Length Research Paper

Evaluation of wheat by polyacrylamide gel electrophoresis

Muhammad Shuaib1*, Mohsin Jamal1, Haji Akbar1, Israr Khan1 and Rehmana Khalid2
  1Department of Biotechnology, University of Malakand, Chakdara, Pakistan. 2Department of Chemistry, Lahore College for Women University, Pakistan.  
Email: [email protected]

  •  Accepted: 03 September 2009
  •  Published: 11 January 2010

Abstract

 

In this study 15 wheat varieties namely Nacozari-76, Ouqab, Tatar-96, Bakhtawar-92, Yecura-70, Raj, Bakkar, Sulilman-96, Pirsabak-05, Maria, Khyber-87, Fakhr-e-Sarhad, Pirsabak-04, Inqulab-91 and Rawal-87 were evaluated for analysis of variability in seed storage proteins by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Electrophorogram for each variety were scored and presence or absence of each band noted and was entered in a binary data matrix. Based on the data of SDS-PAGE gels cluster analysis was performed to check the variations among varieties. The overall result shows low degree of heterogeneity however different varieties reveal differential protein banding pattern. It is concluded that SDS-PAGE analysis of wheat endosperm protein is useful for evaluation of genetic variability and cultivars identification that help in wheat breeding program.

 

Key words: SDS-PAGE, glutenin proteins, wheat varieties, genetic variation and cluster analysis (UPGMA).