African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12484

Full Length Research Paper

Evaluation of the seroprevalence of okra mosaic virus in Koulikoro, Mali

Gaoussou K. KEITA
  • Gaoussou K. KEITA
  • Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA), Katibougou, Koulikoro, Mali.
  • Google Scholar
Laya KANSAYE
  • Laya KANSAYE
  • Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA), Katibougou, Koulikoro, Mali.
  • Google Scholar
Lassina DOUMBIA
  • Lassina DOUMBIA
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali.
  • Google Scholar
Boubacar MACALOU
  • Boubacar MACALOU
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali.
  • Google Scholar
Ibrahim KEITA
  • Ibrahim KEITA
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali.
  • Google Scholar
Mariam SANGARE
  • Mariam SANGARE
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali.
  • Google Scholar
Moussa Noussourou MAIGA
  • Moussa Noussourou MAIGA
  • Institut Economie Rural, Bamako, Mali.
  • Google Scholar
Nadou Paul SANOGO
  • Nadou Paul SANOGO
  • Institut Polytechnique Rural de Formation et de Recherche Appliquée (IPR/IFRA), Katibougou, Koulikoro, Mali.
  • Google Scholar
Ousmane KOITA
  • Ousmane KOITA
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali.
  • Google Scholar


  •  Received: 12 March 2024
  •  Accepted: 11 April 2024
  •  Published: 30 April 2024

Abstract

Mosaic virus disease is transmitted by several types of viruses such as OMD, OYVMV, OLCV, CLCuGV, and CYCrV. Among these viruses, some of them are significant threats to okra production worldwide. To develop an integrated pest management strategy against this disease, this seroprevalence study was conducted at the Rural Polytechnic Institute (RPI) of Katibougou, Koulikoro region, Mali. Symptomatic leaves of okra plants were collected in Koulikoro. The symptoms were mosaic associated or not with other symptoms. For this purpose, 52 symptomatic samples and 3 asymptomatic samples collected in the villages of Niarébougou, RPI Katibougou, and Diakitébougou were analyzed. The DAS ELIZA Okra Mosaic Virus (OkMV) kit was used. Among the symptomatic samples, the analyses showed the presence of 13 positive samples. This indicates a prevalence of 25% in the study area. Okra is infected by the okra virus disease called Okra Mosaic Virus (OkMV), which is the first time in Mali to be detected by the ELIZA test. This study shows the prevalence of the okra mosaic virus in Mali and more precisely in Koulikoro. This study shows the importance of keeping OKMV in consideration among the threats to okra cultivation in this area.

 

Key words: Seroprevalence, okra mosaic virus, Mali.