African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Influence of AgNO3 on somatic embryo induction and development in Manchurian ash (Fraxinus mandshurica Rupr.)

      Dong-mei Kong1, 2, Hai-long Shen1* and Nan Li1          
  1State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin 150040, China. 2College of Life Science, Shanxi University, Taiyuan 030006, China.
Email: [email protected]

  •  Accepted: 02 December 2011
  •  Published: 03 January 2012

Abstract

 

In this present study, we explored the effects of silver nitrate (AgNO3) on somatic embryo induction and the development from immature zygotic embryos in Manchurian ash (Fraxinus mandshurica Rupr.). AgNO3 played a minor role on in vitro embryo induction frequency and in the number of somatic embryos per explant. However, 1 mg L–1 AgNO3 enhanced synchronization and significantly inhibited abnormal somatic embryo formation suggesting that AgNO3 might serve an important function in controlling the development of somatic embryos in Manchurian ash. Our results provided foundation for a future more efficient somatic embryogenesis and regeneration protocol.

 

Key words: Abnormality, Fraxinus mandshurica, silver nitrate, somatic embryogenesis, synchronization.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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