African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Identification of potential fungal strain(s) for the production of inducible, extracellular and alkalophilic lipase

J. Savitha*, S. Srividya, R. Jagat, P. Payal, S. Priyanki, G. W. Rashmi, K. T. Roshini and Y. M. Shantala
Department of Microbiology, Sri Bhagawan Mahaveer Jain College, Center for Post-Graduate Studies, 18/3, 9th Main, Jayanagar 3rd Block, Bangalore 560 011, India
Email: [email protected]

  •  Accepted: 23 January 2007
  •  Published: 05 March 2007

Abstract

In the present study, a total of 32 fungal organisms belonging to 3 different genera were isolated from various sources of which 4 (three Aspergillus sp. and one Mucor sp.) were found to be positive for lipase production. Rhodamine B rapid screening technique was used to determine the lipase activity. Mucor sp. was found to exhibit a maximum fluorescence zone at 350 nm. Among the various types of triglycerides used as the carbon source, sunflower oil was found to be the most effective in inducing lipase inMucor sp. The lipase in Mucor sp. was found to be inducible, alkalophilic and thermostable.

 

Key words: Alkalophilic lipase, extracellular, inducible, Mucor sp, Rhodamine B.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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