African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

Production of thermophilic and acidophilic endoglucanases by mutant Trichoderma atroviride 102C1 using agro-industrial by-products

Mariana Menezes Quadros de Oliveira
  • Mariana Menezes Quadros de Oliveira
  • Centro de Ciências da Saúde (CCS), Instituto de Microbiologia Prof. Paulo de Góes, Departamento de Microbiologia Geral, Avenida Carlos Chagas Filho, 373, Bloco I, Laboratório 055, CEP: 21941-902, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
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André Luiz Grigorevski de Lima
  • André Luiz Grigorevski de Lima
  • Centro de Ciências da Saúde (CCS), Instituto de Microbiologia Prof. Paulo de Góes, Departamento de Microbiologia Geral, Avenida Carlos Chagas Filho, 373, Bloco I, Laboratório 055, CEP: 21941-902, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
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Elba Pinto da Silva Bon
  • Elba Pinto da Silva Bon
  • Centro de Ciências Matemáticas e Natureza (CCMN), Instituto de Química, Departamento de Bioquímica, Avenida Athos da Silveira Ramos, 149, Bloco A, sala 539, CEP: 21941-909, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
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Rosalie Reed Rodrigues Coelho
  • Rosalie Reed Rodrigues Coelho
  • Centro de Ciências da Saúde (CCS), Instituto de Microbiologia Prof. Paulo de Góes, Departamento de Microbiologia Geral, Avenida Carlos Chagas Filho, 373, Bloco I, Laboratório 055, CEP: 21941-902, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
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Rodrigo Pires do Nascimento
  • Rodrigo Pires do Nascimento
  • Centro de Tecnologia (CT), Escola de Química, Departamento de Engenharia Bioquímica, Avenida Athos da Silveira Ramos, 149, Bloco E, sala 108, CEP: 21941-909, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
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  •  Received: 23 September 2015
  •  Accepted: 19 January 2016
  •  Published: 16 March 2016

Abstract

Many traditional mutagenic strategies have been used to improve cellulase production by micro-organisms, especially fungi species. Trichoderma species are among cellulolytic fungi, those that have been most extensively studied, due to their efficient production of these enzymes. In the present study, N-methyl-N´-nitro-N-nitrosoguanidine (NTG) was used as mutagenic agent to obtain cellulolytic mutant from wild strain T. atroviride 676. After mutagenic procedures, two strains (102C1 and 104C2) were selected as promising cellulase-producing mutant. The effect of the carbon (sugarcane bagasse: SCB) and nitrogen (corn steep liquor: CSL) sources on endoglucanase production by the mutants 102C1 and 104C2 was studied using submerged cultivations at 28°C. Different concentrations of SCB and CSL were used and nine different media were generated. Mutant 102C1 showed the best results when using 2.5% SCB and 0.7% CSL. A central composite rotational design (CCRD) was performed to estimate optimal conditions of pH and temperature for endoglucanase activity of strain 102C1, which were pH 3.6 and temperature 66°C. The characterization of this acidophilic and thermophilic endoglucanase activity produced by the mutant strain 102C1 allows its use in biotechnological applications, particularly in the hydrolysis of agro industrial residues, such as SCB, for bioethanol production.

 

Key words: Trichoderma atroviride, CMCase, nitrosoguanidine, sugarcane bagasse, corn steep liquor.