African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic characterization of two traditional leafy vegetables (Sesamum radiatum Thonn. ex Hornem and Ceratotheca sesamoides Endl.) of Benin, using flow cytometry and amplified fragment length polymorphism (AFLP) markers

Adéoti, K.1,2, Rival, A.3, Dansi, A.2*, Santoni, S.4, Brown, S.5, Beule, T.3, Nato,A.6, Henry, Y.6, R. Vodouhe7, Loko, L. Y.2 and Sanni, A.1
1Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi (UAC), BP 526 Cotonou, Republic of Benin. 2Laboratory of Agricultural Biodiversity and Tropical Plant Breeding, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi (UAC), 071BP28, Cotonou, Republic of Benin. 3CIRAD, UMR DIADE, IRD, UM2. 34398 Montpellier Cedex 15, France. 4INRA, UMR AGAP, Unité de Génétique et Amélioration des plantes, Atelier de Marquage Moléculaire, 34060 Montpellier Cedex 01, France. 5CNRS, UPR2355, 91198 Gif-sur-Yvette, France. 6Institut de Génétique et Microbiologie, Bâtiment 360, Université Paris-Sud 11, 91405, Orsay, France. 6Institut de Biotechnologie des Plantes, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay, France. 7Bioversity International, Office of West and Central Africa, 08 BP 0931, Cotonou, Republic of Benin.
Email: [email protected]

  •  Accepted: 13 July 2011
  •  Published: 24 October 2011

Abstract

Amplified fragment length polymorphism (AFLP) markers and flow cytometry were applied for the genetic characterization of wild and cultivated accessions ofSesamum radiatum and Cerathoteca sesamoides; two neglected and underutilizedspecies of traditional leafy vegetable consumed in Benin. The average 2C nuclear DNA content per nucleus was found to be 1.99 ± 0.06 and 1.05 ± 0.06 pg for S. radiatum and C. sesamoides, respectively which correspond to estimated genome size of 1946,22 Mpb for S. radiatum and 1026,9 Mpb for C. sesamoides. No variation in DNA content could be detected within accessions from each analysed species. Also, no relation was found between nuclear DNA content, ecogeographical origin and the status (cultivated or wild) of the analyzed accessions. AFLP markers revealed low diversity within the accessions analyzed. Results from the study contributed to a better characterization of S. radiatum andC. sesamoides accessions and will help in defining both genetic resources conservation and breeding strategies.

 

Key words: Amplified fragment length polymorphism (AFLP) markers, Ceratotheca sesamoides, flow cytometry, genetic diversity, ploidy, Sesamum radiatum, leafy vegetables.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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